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The biosensor was designed by modifying the surface of a carbon screen-printed electrode with platinum nanoparticles and reduced graphene oxide, followed by the laccase drop-casting and stabilization in neutralised 1% Nafion solution.
Fishes were preserved in neutralised and buffered 10% formalin for ten days and then transferred to 70% alcohol.
A range of glycogen standards were prepared in neutralised PCA containing 0 to 300 µg of bovine liver glycogen (Sigma Aldrich) in a 200 µl volume.
Finally, LPS was visualized by 12.5% SDS-PAGE [ 17]. S. mutans TA or LTA was resuspended in neutralised distilled water and put in contact with the coupled resin at 37°C for 2 h.
Cell recombinants were prepared by mixing 100,000 epithelial (BPH1) cells with 250,000 CAFs in neutralised rat tail collagen prepared as described previously (Ao et al., 2007; Olumi et al., 1999).
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For histology, tissue samples were fixed in 10% neutralised formalin and embedded in paraffin.
Froome and Sky had their hiccups but he and his team rode their luck, as all Tour winners do, with his only crash a pratfall in the neutralised zone of the first stage in Corsica.
"I had to have a little gulp in the neutralised zone," she said of the start in Otley.
The peloton rode north out of Leeds in a neutralised procession before lining up once again for the official start of the stage to Harrogate.
And if the Freudians (and Jonathan Swift) are right that civilisation is the distance Homo sapiens ("Yahoos") puts between his nose and his excrement, I am a more civilised person for living in my neutralised world.
After 2 hours of starvation, followed by a 2-hour incubation period with the inhibitor, the cells were lysed and ATP and lactate levels were determined in the neutralised lysates (Figures 4 and 5, respectively).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com