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The gel was removed from the cassette, washed extensively in multiple changes of water and stained with Gelcode Blue® Coomassie stain (Pierce/Thermo Fisher).
In simulated evolution of populations of networks, single mutations in regulatory or coding regions resulted in multiple changes in regulatory connections among genes, or alternatively in neutral change that had no effect on phenotype.
IBV Beaudette is a well known apathogenic lab strain of IBV that was attenuated by multiple, several hundred, passages in 11-day-old embryonic chicks [30], therefore loss of virulence could have resulted in multiple changes throughout the genome.
AFEX is a complex physiochemical pretreatment, resulting in multiple changes to the cell wall structure.
Overall, the in silico approach used herein indicates that, once ITGA6 gene is knocked-down, several intracellular circuits comprising the expression of membrane and secretory moieties related to cell adhesion and/or cell migration (e.g. integrins, ECM and chemokines) by human TEC may be altered, resulting in multiple changes in cell behavior.
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In contrast, multiple changes in miRNA abundance can occur in vivo [ 32], where simultaneously up-regulated and down-regulated miRNAs can target the same gene but with a range of predicted efficacies [ 25].
This flexibility in work re-design seemed necessary in promoting multiple changes linked with a reduced musculoskeletal risk.
In conclusion, the lack of change in the insulin receptor number in combination with multiple changes in post receptor signaling suggests a post receptor defect.
In recent years, multiple changes in diabetes care have been introduced in the Netherlands, with the aim of improving the continuity and quality of care.
A mutant protein implicated in disease can have multiple changes in its behavior in the network.
The decrease of K⊥, K//, and MK values in IGA subjects indicated multiple changes in brain microstructure, which may be implicated in the underlying pathophysiology of IGA.
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