Our results show that in MPCs optimized for light intensity modulation applications, in the red spectral region (near 650 nm), the achievable levels of optical transmission are limited to about 30%.
Here, we first tested if 9CRA and ATRA can induce osteogenic differentiation in MPCs.
Collectively, these results demonstrate that 9CRA and ATRA can effectively induce the early osteogenic marker ALP activity in MPCs.
These results strongly suggest that retinoic acids and BMP9 act synergistically in promoting osteogenic differentiation in MPCs.
By contrast, the DBDM could induce IGFBP3 suggesting that in MPCs EWS-FLI-1 does not require integrity of the DBD for IGFBP3 upregulation.
When the effects of the two FUS-ERG fusions in MPCs were compared, all of the genes tested displayed movement in the same direction.
In contrast to the limited but nevertheless significant overlap of induced and repressed transcripts in response to the three fusion proteins, ERG-1 and FLI-1 shared very few common targets in MPCs.
Among potentially relevant genes found to be induced in MPCs but not in other primary cells infected with EWS-FLI-1-containing vectors was IGF-1, which constitutes a key growth factor for ESFT survival [8].
