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Laser manufacturers are nowadays driven by the wide variety of laser applications to build compact and robust devices that are able to perform in more stringent conditions [1].
Since we showed that the clones selected above could bind specifically to C. albicans cells, we decided to test the specificity of interaction in more stringent conditions.
This assay was therefore chosen among other cell-based assays in order to test molecules in more stringent conditions and maybe avoid subsequent disappointing results in vivo [25], [26].
When I-BAR-expressing cells were extracted in more stringent conditions in the absence of PEG (Fig. 5), the GFP-I-BAR fluorescence (Fig. 5A, insets) and corresponding submembranous material were largely removed exposing the structure of the underlying cytoskeleton (Fig. 5).
Our analysis were performed in more stringent conditions than that of Ambroset et al.[ 4]: the amounts of ergosterol and oleic acid used were half those generally used (the final concentrations were 7.5 mg/l for ergosterol and 2.5 μl/l for oleic acid) and fermentations were performed at 24°C.
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However, their optimal exponents are always smaller than the corresponding exponents in the whole space, leading to more stringent conditions in the applications (to blow-up estimates).
This was performed to identify shorter fragments of sequences belonging to the same family that were included in different clusters when more stringent conditions were applied.
Proposition 1 and Proposition 2 convey our most important result, namely that the simultaneous game increases total contribution, but it requires more stringent conditions in order to get positive contributions from both players.
Thus, there is a need to conduct a rigorous trial of pure ACT for psychosis to test whether the promise shown in initial trials holds under more stringent conditions.
If similar studies are conducted under more stringent conditions in the future, a study design that includes third party assessments by physicians and other pharmacists using predefined criteria would be desirable.
"Firms in Europe have tended to put more stringent conditions on long-term incentive awards than in America," says Richard Bednarek, global director of executive remuneration for Hay Group.
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