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In presence of doxycycline, MIF shRNA elicited a 90% decrease in MIF mRNA expression.
The level and pattern of MIF expression show a general increase in MIF levels associated with disease progression.
To elucidate the role of MIF in MMP-2 production, we produced zymosan-induced arthritis (ZIA) in MIF gene-deficient and wild-type mice.
It is suggested that CD44 functions in MIF signal transduction in some cells via horizontal recruitment of the protein into the MIF-CD74 complex [ 20].
Also the warping of section takes place in MIF and FEM analysis.
Figure 9 Schematic of SPR electric field localization (lateral component) in MIF for different dielectric cover thicknesses.
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We next explored a possibility that p53 is involved in MIF-induced HIF-1 activation.
Since we have demonstrated that β-arrestin1 is involved in MIF-initiated sustained ERK activation, we further investigated the role of β-arrestin1 in MIF-induced cell cycle progression.
Besides CD44 and CD74, MIF has another two cell surface receptors, chemokine receptor CXCR2 and CXCR4, which are involved in MIF-mediated migratory function [5].
Because it was reported that MIF induced angiogenesis in a MAPK-dependent manner [27], we examined involvement of MAPK in MIF-elicited HIF-1 activation.
These data imply but do not demonstrate a role of CD74 in MIF-dependent inflammatory responses.
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