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Three 1-sec tetani were simulated (stimulus parameters in Methods) with 5-min intervals between tetani.
Immunohistochemistry studies of various human and mouse tissue sections were carried out as described in Methods, with the control IgY in comparison.
The concentration of bacteria in the spiked samples was chosen to be at a level that would allow quantification of the recovery in methods with high and low recovery.
To allow a comparison, we simulated populations as described in Methods with N = 200, L = 20, A = 8, and drew samples after 10 generations – long enough for levels of LD to stabilize.
Ct values were normalized to the expression of the housekeeping gene beta-actin (ΔΔCt), and fold difference in mRNA calculated as 2ΔΔCt, as detailed in Methods, with p ≤ 0.05 considered significant.
Using these values of α, we then predicted DMP in the Taiwanese population of C. vestalis, for an sl-CSD and a two-locus CSD scenario (see formulae 2 and 3 in methods), with sex allele diversity between 3 and 100 per locus.
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The model is based on particle-in-cell methods with Monte Carlo Collision techniques for modeling of collisions.
Declines in dominant methods with increases in other methods toward a balanced method mix.
Bangladeshi researchers trained in qualitative methods with expertise in eliciting responses about sensitive topics led the group discussions.
Aliquots were digested as in methods, except with 0, 0.5, 1, 2, 3 or 4 U MNase/μg total RNA.
When the protocol was unavailable, we compared outcome definitions in the methods with those reported in the results.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com