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This is one step in method developments that will close the gap to more invasive techniques, such as atomic force and electron microscopy.
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The prostate cancer cell lines served a reference in method development because the ss-qPCR method was previously developed with these cell lines [ 6, 31, 35].
Collaborating with Claudia Tesche and Riitta Hari he was in engaged in method development and memory research.
We thank Anna Francis for assistance in method development for histopathological assessment.
DoE identify significant factors and then optimise a response with respect to them in method development.
This approach significantly reduces the number of runs in method development.
Extraction efficiencies of analytes should always be tested in method development, so that spike recovery can really represent the accuracy of analytical methods for medicinal herbs.
It requires only four experiments on standard equipment, is based on straightforward calculations, and is therefore easy to use in method development.
The use of experimental design in method development was studied for the chiral separation of several amino acid derivatives with capillary electrophoresis.
Two uses of DoE in chromatography are for showing lack of significant effects in robustness studies for method validation, and for identifying significant factors and then optimising a response with respect to them in method development.
The next step in method development was to quantify the degradation rate of the test substances.
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