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Adopting sleeping mode in MBSs.
On the other hand, only adopting sleeping mode in MBSs effectively reduces system energy consumption while reduces the network coverage.
For adopting sleeping mode in MBSs, some researches verify that energy consumption of MBSs can be significantly reduced while maintaining the downlink coverage and user power consumption performance [10].
For spectral and kinetic spectrofluorometer experiments (FluoroMax 3, Horiba Jobin-Yvon, Edison, NJ, or PC-1, ISS, Champaign, IL, USA), an aliquot of cells (usually between 0.4×10 and 3.0×10 cells/mL in MBSS) was transferred to a quartz fluorometer sample cell and allowed 5 min to equilibrate.
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Cells were bathed in isotonic MBSS buffer and 10 to 20 cells of interest were selected using a freehand ROI (Regions of Interest) drawing tool.
HeLa-TRPV4 cells grown on glass coverslips were loaded with 2 µM fura-2/AM for 1 hr at RT, then washed in the isotonic MBSS buffer (containing 140 mMNaCl, 5.4 mMKCl, 0.5 mM MgCl2, 0.4 mM MgSO4, 3.3 mM NaHCO3, 2 mM CaCl2, 5.5 mM glucose, 10 mM HEPES, pH 7.4).
Ionomycin, MC540, paclitaxel, and actinomycin D were dissolved in dimethylsulfoxide (DMSO); TMA-DPH in dimethylformamide; methotrexate in slightly basic modified balanced salt solution (134 mM NaCl, 6.2 mM KCl, 1.6 mM CaCl2, 1.2 nM MgCl2, 18.0 mM Hepes, 13.6 mM glucose, pH 7.4, MBSS); daunorubicin in water.
The purpose of MBSs is to maintain network coverage, meanwhile MBSs increase energy consumption tremendously.
The mobile users (UEs) in the network are divided into two types, i.e., UEs that communicate directly with MBSs (M-UEs) and UEs that communicate with MBSs via the help of RSs (R-UEs).
"Come in, come in!
"You in?
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