Pooled oocytes were matured in maturation medium for 24 h in 200 µl within a Nunc 4-well dish (Nunclon, Roskilde, Denmark), then inseminated in BIVF medium (200 µl, 2 × 10 / ml final sperm concentration).
These genes encode the NadAC proteins, involved in NAD synthesis, a putative cysteine desulfurase, possibly involved in maturation of FeS clusters necessary for function of the NadAC complex, and the regulator NrtR [ 8, 31].
Consistent with a role for Rpn12 in maturation of the assembling lid, 2D class averages indicated that Rpn12 addition caused extension of Rpn6-N to a conformation similar to that observed in the purified full lid.
BM-MSCs were induced to hepatogenesis in differentiation medium for 2 weeks and incubated in maturation medium for another 2 weeks.
The COCs were cultured in maturation medium for 1 h (1 + 23 h), 12 h (12 + 12 h) or 24 h in the presence of follicular cells and the respective treatments and for the remaining period in the absence of follicular cells to complete 24 h.
Thus, the objectives of this study were to describe temporal changes in maturation sizes for chum salmon before and after the closure of the high seas gillnet fishery.
Development of polyamine and biogenic amine systems in brains and hearts of neonatal rats given dexamethasone: role of biochemical alterations in cellular maturation for producing deficits in ontogeny of neurotransmitter levels, uptake, storage and turnover.
COCs were then placed in maturation medium and matured for 16 hours in a volume of 50 μl medium/COC at 37 °C under paraffin oil, in humidified air comprised of 20% O2, 6% CO2 and N2 balance.
We hypothesized that the transmission ratio distortion of the Shb null allele reflected abnormalities in oocyte maturation, for which we found evidence in the present study of Shb deficient mice.
After in vitro maturation for 24 h, the oocytes were randomly allocated into three groups: (1) untreated (control), (2) exposed to vitrification solution (VS) without being plunged into liquid nitrogen (toxicity), or (3) vitrified by open-pulled straw method (vitrification).
