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Some of Hartmann et al.'s [ 39] EST-like patterns of missing data are reminiscent of this case because of blocks of missing data at the ends in many alignments.
The presence of truncated sequences in many alignments meant that the exclusion of gap sites sometimes ruled out the entire alignment (i.e., when all alignment columns had at least one gap).
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Second, genome rearrangements disrupt the synteny implicit in many alignment procedures.
This gives only a rough upper bound on the time required if a pipeline was designed from the ground up to use both methods; since the majority of sequences are identified in common by both approaches, many alignments are unnecessarily performed multiple times, in addition to the simple overhead of running two searches and combining the results.
In addition, this mode of alignment was combined with options that define which and how many alignments should be reported; the option "-k 1 --best" instoucted bowtie to report only the best alignment if more than one valid alignment exists, while the option "-m 1" instructed bowtie to refrain from reporting any alignments for reads having multiple reportable alignments.
Alignments with low scores are typically pairs with low frequency where the source and target words occur in many other alignment pairs.
Magnetically aligned bicelles represent one of the many alignment media used in the study of RDCs.
We considered an alternative approach of using the orthologous sequences present in the multiple sequence alignments discussed above, but in many cases the alignments contained < 1 kb of orthologous sequence concentrated around the TSS, and in some cases aligned sequences comprised several non-contiguous pieces of the orthologous genome.
The idea would be that if a protein complex is conserved in many species, the PPIs in this complex are likely to be conserved as well, and therefore the corresponding subgraph in the APE graph should contain many alignment extension links.
Only alignments in which many aligned node pairs perform the same function should be used to transfer function from annotated parts of one network to unannotated parts of another network [ 30].
While many alignment methods focus on detecting remote homologs in order to expand coverage of functional inference, obtaining high-quality alignments remains difficult even for closely-related families.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com