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The exclusion of non-English papers resulted in low representation of experience from francophone Africa and Latin America, and there may also have been material in the grey literature that was not examined.
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Interestingly, numerous bacteria belonging to the genera Klebsiella were found when larvae were fed on barley but were absent in larvae from the artificial feed or only found in very low representation in lima bean-fed insects, which could indicate an important role for species of this genera in larva feeding on grass species, as was seen in our study on sugarcane.
Additionally, small fragment libraries (typically <400 bp inserts) that are commonly used for paired end sequencing result in a low representation of mapped read pairs that successfully span either end of the transgene insertion.
Dideoxy-sequencing still remains the golden standard for DNA analysis, although mutations may be missed in cases with low representation of tumor DNA, as exemplified in two cases in this study.
In contrast, BTSAT4 showed low representation in the HELP cocktail libraries (0.9% and 0.5% for Day-7 and Day-12 respectively) (Additional file 1: Table S2).
While many alleles found in East Africa were not addressed in the current assay, their very low representation in these populations results in their relatively low public health impact.
This deficiency is reflected in the very low representation of East African populations in global HLA databases [51].
Errors in sequencing and the assembly of contigs could have resulted in the apparently low representation of full-length transcripts.
Taken together, this implies that two divergent forms of PIV SVMPs may be uniquely present in E. coloratus, despite their low representation in this species.
The study was conducted predominately in high-income countries (i.e. United States and western Europe) and had requirements for frequent visits, which resulted in a relatively low representation of women and nonwhite participants.
Although amplification with nonspecific 16S rRNA gene primers has been used to characterize environmental communities with high abundance of anammox bacteria (e.g., anammox bioreactors), in samples with low representation of anammox, nonspecific 16S rRNA clone libraries are not a good choice.
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