Sentence examples for in logarithmic phase from inspiring English sources

Bacillus thuringiensis grew on quinalphos with a generation time of 28.38 min or 0.473 h in logarithmic phase.

The E. coli and S. aureus in logarithmic phase were washed with aquae sterilisata for three times, and then the concentration of the bacterial suspension was adjusted to 107 CFU/mL.

The optimal transformation efficiency was approximately 101 ± 7 transformants per μg plasmid and was obtained under the following conditions: 5 × 106/plate cell density in logarithmic phase; 30 μg/mL plasmid; 660 V pulse voltage; 3.5 ms pulse width; and 30 μg/mL G418.

All Leishmania studies were performed with promastigote (insect stage) in logarithmic phase of growth.

Promastigotes in logarithmic phase of growth were harvested by centrifugation (1000×g, 10 min, 4°C), washed twice with HPMI.

GBS grew rapidly in this medium, with a generation time of ∼35 min in logarithmic phase (Fig. 1).

Briefly, HepG2 transfectants in logarithmic phase were harvested, fixed in ethanol, and incubated with 0.5 mg/ml of propidium iodide (PI) along with 0.1 mg/ml of RNase A (200 KU; Calbiochem, San Diego, CA), prior to cell-cycle analysis.

To visualize endogenous PE on the cell surface, 5×106 promastigotes in logarithmic phase of growth were incubated in 20 µl HPMI containing 38 µM biotinylated Ro09-0198 (provided by Kazuma Tanaka).

Infections were carried out using cultures of O. anthropi or B. abortus in logarithmic phase diluted in Eagle's minimal essential medium to reach the desired multiplicities of infection (MOI).

Finally, oligonucleosomal DNA degradation could only be observed in the population enriched for metacyclic promastigotes, but not in logarithmic-phase promastigotes (Fig. 5A).

To disrupt the actin cytoskeleton in logarithmic-phase cultures, latrunculin A (Santa Cruz Biotechnology, Dallas, TX) was used at a concentration of 10 µM in SD medium lacking leucine (SD-Leu), following the procedure of Hammermeister et al. (2010).