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In order to minimize losses attributable to a fail in locate the patient, up to six telephone calls will be made at different times and on different days; after six failed calls, will try to contact the patient by the general practitioners.
Of these, 35 families, ranging in size from 4 to 10 individuals, could be analyzed in LOCate on 1.7 GB-memory instances on the Amazon cloud [34].
After matching identifiers in LOCATE to UniProt ACs and removing LOCATE-specific annotation terms, 1713 (of 2068 original) proteins and their locations (LOCSCL) remained.
As LOCATE is not cited as literature for any protein in UniProt, we expect the SCL information in LOCATE to be independent of UniProt annotation.
Manual annotations were complemented by sequence-based predictions in eSLDB (26) and further by experimental data sets in LOCATE (27), locDB (28) and SUBA3 (29).
In LOCATE, the SCL data generated by experimental investigation are clearly separated from SCL annotation extracted from the literature, and it is the experimental SCL data that we use in the validation presented here (Section 9).
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