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There has been tremendous success in developing and using fluorescent probes with new characteristics in FLIM.[ 4] However, disadvantages of current standard fluorescent probes with short emission lifetimes are that 1) they emit on the same timescale as background autofluorescence; and 2) the variations in lifetime required for FLIM purposes are relatively small.
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I READ recently that the construction of Princess Diana's wedding gown, one of the more elaborate get-ups anyone has worn in my lifetime, required 25 yards of silk taffeta, 100 yards of tulle and at least 10,000 mother-of-pearl sequins and beads.
The rapid social changes that were taking place in his lifetime required new institutions, as traditional customs and laws proved themselves unable to cope with such issues as mass immigration, class conflict, the Great Depression, the demands of women for greater independence, and the threats to democracy posed by fascism and communism.
Interstellar transportation over periods shorter than the human lifetime requires speeds in the range of 0.2 0.3c.
The ever increasing demands for high precision machining and increased cutting performance, in terms of cutting speed and lifetime, require wear resistant tools of large dimensional accuracy that have very sharp cutting edges.
In addition, accurate determination of fluorescence lifetime requires the detection of a large number of photons.
Application of this material in automotive fuel cells and targeted lifetimes require consideration of time-dependent failure of Nafion® in fuel cell design.
Further analyses with complementary methods sensitive to impurities which could account for differences in lifetime would be required to better refine these hypotheses.
To assess the safety of an existing bridge, the loads to which it may be subject in its lifetime are required.
All PIs train many more postdocs in their lifetimes than required to replace themselves, and new faculty positions are not increasing at anywhere near the rate required to compensate for this.
The Republican measure would transfer authority over much of the safety net for the poor to the states, limit a recipient's welfare benefits to five years in a lifetime and require work after two years on the rolls.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com