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The best fit for cells in LatA is obtained for 10% active actin.
However residual actin polymerization may have persisted in these cells, since a For3p mutant (I930A) that cannot bind to actin barbed ends had undetectable recovery in LatA [4].
(B ) The orientation of GFP-Sla2-RFP molecules at endocytic sites in LatA treated cells.
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Phosphorylation of Ypk1 T662 was monitored at the indicated time points following PalmC treatment in LatA-treated cells.
His chief architectural works of this period were the facades of Santa Maria della Pace (1656 57)—perhaps his most ingenious conception and Santa Maria in Via Lata in Rome (1658 62).
Silvestro e Martino, Guido of S. Maria in Trastevere, Imar of Tusculum, Raymond of S. Maria in Via Lata and Simeone of S. Maria in Domnica Guglielmo of S. Pietro in Vincoli was probably the seventh one.
Of particular interest are the CYP65s which were more abundant in the ascomycete trunk pathogens (e.g. 34 genes in N. parvum and 34 in E. lata) compared to B. cinerea (11) and were not detected in the two basidiomycete analyzed and S. cerevisiae.
AA3s were expanded the most in N. parvum, and AA9s in E. lata and Dia.
In contrast, t3PKS clusters were found only in E. lata, T. minima, Dia.
Glycoside hydrolases (GHs) represented the largest superfamily, ranging from 76 genes in P. chlamydospora up to 195 genes in E. lata.
In addition, LatA treatment had no effect on non-phosphorylated, or phospho-CTD levels in either repressing or non-repressing conditions (Figure 4B).
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