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Indeed, this single mutation lowered the methanol concentration for which the initial rates of alcoholysis and hydrolysis are equal from 2 M in CAL-A down to 0.3 M in its mutant, while the exceptional stability of the parental enzyme toward alcohol and temperature was conserved.
EMSAs were performed based on a previously established protocol [46] using an oligonucleotide encompassing the Stat-binding site of the mouse Cdkn1b promoter in wild type form (5'-TTAATTTCCTGTAACATG-3') or in its mutant form (5'- TTAATTGTCTGCGACATG-3'; mutations are underlined) as reported [18].
In its mutant form, the enzyme does not function.
To elucidate the genome-wide effects of PTK7 in its mutant and truncated forms, we performed a whole genome transcriptional analysis of the cells.
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A similar increase in plant height was observed in N22 and its mutant NH219 under heat stress and the increase was more in the mutant.
A parallel experiment in MCF7 cells showed that, in contrast to its mutant counterpart, the levels of WT p53 were increased by STCA.
Another member of the carboxypeptidase family, carboxypeptidase E (CPE) has been reported to cause proinsulin processing defect in its mice mutant (Naggert et al., 1995).
In this report, we describe an integrated analysis of experimental data from a study of the transition from aerobic to anaerobic growth in the wild-type strain of MR1 and in its Crp mutant.
OsDVR encodes a functional 8-vinyl reductase and can convert divinyl Chl a to monovinyl Chl a in rice, and its mutant exhibits a yellow-green leaf phenotype (Wang et al. 2010).
The BK channel encoding mSlo α-subunits alone shows no rectification in currents, but its mutant I323T exhibits an apparently "outward-rectified" current.
Expression of Arabidopsis AtCCX3 and AtCCX4 in mutant yeast suppressed its mutant defective phenotypes in Na+, K+, and Mn2+ transport [ 84].
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CEO of Professional Science Editing for Scientists @ prosciediting.com