Sentence examples for in its medium from inspiring English sources

After 4 days in culture, EBs were plated on matrigel-coated (BD Biosciences) plates in ITS medium: DMEM F12 (Life Technologies), ITS supplement (Sigma) and penicillin/streptomycin (Lonza) for about 16 days.

Cell clumps were collected and replated on matrigel-coated plates in ITS medium.

For the induction of neural differentiation, the immortalized hMSCs were treated with several neurogenic factors in ITS medium that was prepared as described previously [19].

In some experiments, 500 ng/ml recombinant Noggin (R&D Systems) and 10 µM SB431542 (Tocris) were added at the onset of the experiment and continued through day 10 in ITS medium.

After comparing the migration pattern of 3 control and 3 FD hOE-MSCs in serum medium and serum-free medium (ITS), we determined that FD cells invasion is significantly reduced compared to control cells both in serum and in ITS medium (Figure 5).

Cultures enriched with human neurons were derived by terminal differentiation of H9 hES-NSCs for 10 days in ITS medium (insulin concentration, 0.22  μM) and supplemented with brain-derived neurotrophic factor (BDNF; 20 ng/ml; R&D Systems), glial cell-derived neurotrophic factor (GDNF; 20 ng/ml; R&D Systems), and dibutyryl cAMP (0.5 mM; Sigma) and ascorbic acid (0.2 mM, Sigma).

"The way we see it is that every generation builds brands in its mediums," he says.

But the average space devoted to food in its medium-sized stores is about 7,000 sq ft and it is understood that there are dozens which could be expanded by up to 30%.

Watts said the umbrella organization had three main objectives in its medium-term strategy.

"Economic slack persists and inflation is running below the Fed goal in its medium-term projection," Mr. Reinhart wrote.

It was a key success and now this forecasting prowess is to be further exploited by the Reading centre – rated one of the world's top forecasting organisations – in order to make further improvements in its medium-range forecasting, which typically covers weather events over three to 10 days.