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The first point in ITC titration was not included in fitting.
Altria, the world's largest cigarette maker, holds, through Philip Morris International, a 36% stake in ITC rival Godfrey Philips.
Highly purified EV71 mature virions and the synthesized peptide (aa 146 166 of SCARB2) were in ITC buffer (PBS buffer pH 7.4).
An optimized inhibitor (compound 17) exhibited potent Hsp90 inhibition in ITC, biochemical, and cell-based assays (Kd = 1.3 nM, Ki = 15 nM, and cellular IC50 = 0.5 μM).
The proposed method can be used to estimate the heat of dilution and is therefore robust towards this ubiquitous error in ITC.
Lowering of the electron-transfer activation energy by water is assigned in part to release of molecular rigidity but in ITC the major role is assigned to a nucleophilic attack of water already in the activated state.
Since Baba Jukwa started spilling secrets, the government has brought in ITC experts from China and Israel, and employed an Australia-based hacker/spy to try to identify the self-described saboteur.
All peptides were dissolved in ITC buffer.
The adenosine molecules, ATP, ADP and AMP did not show any appreciable heat changes in ITC.
A solution containing fluorescein labelled peptide1 (0.5 µM) was prepared in ITC buffer.
Such co-solvents are typically not included in ITC experiments because of extreme dilution heats if the buffers are not precisely matched.
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