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Subsequently we carried out a sensitivity analysis to determine the influence of missing data on smoking status on the study conclusions, varying the strength of association between smoking status and "missingness" (that is, whether data are missing), and using multiple imputation to allow for variation in individual sampling and imputation.

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They show a wide range of reflectance values (discussed below), but in individual samples these values tend to be intermediate compared with those of the other maceral groups.

The results indicated the differences in protein pattern in individual sample.

Western blotting was performed to confirm PSMA7 expression in individual samples.

Nitrosomonadaceae (uncultured) presented a relative abundance varying from 0.27 to 3.8% in individual samples.

The most abundant genus is Nitrospira with a relative abundance varying from 0.8 to 6.1% in individual samples, while the second is Gemmatimonadaceae (belonging to Gemmatimonadetes phylum) with a relative abundance varying from 1.1 to 3.6% in individual samples.

Guanidine-insoluble material was rendered soluble by papain digestion, to enable quantification of collagen, residual glycosaminoglycan, and residual unextracted DNA in individual samples.

Three of these candidates were validated by qPCR in individual samples, but none of these three could be confirmed in later studies.

Since pinwheel densities can be measured in individual sample maps thanks to the ergodicity properties of invariant Gaussian fields (see [38], Sect.

Therefore, a statistical model was constructed based on the multiple parameters such as distribution of proteins in individual sample, between biological or technical replicates.

The mRNA expression was normalized to the expression of GAPDH in individual samples.

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