Abundances in individual samples were calculated by adding the single abundances of the respective peptides.
After excluding PMDs, a hidden Markov model was applied to identify UMRs and LMRs in individual samples (Fig. 2a).
This band was absent in individual samples, and, therefore, could only correspond to the heterocomplex trapped by the F299C K102C bond (Fig. 6e).
The x-axis shows qRT-PCR expression averages (−ΔCt) over GZMH, GNLY, FGFBP2, and GZMA genes in individual samples, while the y-axis shows the same summaries derived from high-throughput expression profiling methods.
They show a wide range of reflectance values (how the coal reflects light; discussed below), but in individual samples these values tend to be intermediate compared with those of the other maceral groups.
Guanidine-insoluble material was rendered soluble by papain digestion, to enable quantification of collagen, residual glycosaminoglycan, and residual unextracted DNA in individual samples.
They show a wide range of reflectance values (discussed below), but in individual samples these values tend to be intermediate compared with those of the other maceral groups.
Western blotting was performed to confirm PSMA7 expression in individual samples.
Nitrosomonadaceae (uncultured) presented a relative abundance varying from 0.27 to 3.8% in individual samples.
The most abundant genus is Nitrospira with a relative abundance varying from 0.8 to 6.1% in individual samples, while the second is Gemmatimonadaceae (belonging to Gemmatimonadetes phylum) with a relative abundance varying from 1.1 to 3.6% in individual samples.
Three of these candidates were validated by qPCR in individual samples, but none of these three could be confirmed in later studies.
