The cells were then washed with fresh medium, incubated for indicated time in fresh medium, and suspended in 200 µl of radio-immunoprecipitation assay (RIPA) buffer.
(D ) Huh-7 cells transfected with hNTCP were infected with HDV similarly as in panel B. The HDV viral RNAs in infected cells at indicated time points were quantified by real-time RT-PCR.
(E) Immunoblot of BMAL1 recovered from immunoprecipitations of HDAC5 from primary hepatocytes exposed to FSK. (F) Immunoblot showing effects of H89 treatment on FSK-induced BMAL1 phosphorylation at indicated time in primary hepatocytes.
In some experiments, at indicated time points after inoculation of tumor cells recipients were injected intravenously with 5×106 PBS or α-MSH stimulated CD8+ cells.
To examine this idea, we determined mRNA levels of PRPF3 in mouse tissues at indicated time points via quantitative RT-PCR.
(A ) GST-CTD proteins were phosphorylated by Abl tyrosine kinase for indicated time in vitro.
Results are present as mean and standard deviation from three dogs in the indicated time points.
Results are presented as means and standard deviation of three different dogs in the indicated time points.
Samples of 250 mL were collected in the indicated time points (0, 2, 6, 30, 96 hr) after exposure to DD treatment.
In the indicated time periods, 0.1 ml of spent medium was replaced with an equal volume of fresh medium containing MTT 0.5 mg ml−1.
HeLa, SK-HEP-1, SW480, and PC-3 cells were treated with G-Rh2 (7.5 μg/mL) in serum free media for indicated time periods and then were harvested.
