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Cells containing distinct ThS signals to indicate the presence of the aggregated material with β-pleated sheets were scored in independent fields containing a total of 300 cells [33].
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To test this assumption, we counted InsR/TH superimpositions in 42 independent fields in three NAc immunolabelled sections from two rats.
The stained cells were counted in five independent fields in each Transwell.
The number of positive staining cells was counted in five independent fields in random areas on two non-consecutive slides at 200× magnification.
Twelve hours later, images of the wound were taken using a 10× objective in an OLYMPUS IX51 microscope equipped with an OLYMPUS camera (OLYMPUS, Center Valley, PA USA) and cells in the wound area in five independent fields were counted.
In the immunohistochemistry experiments, all PCNA positive nuclei were counted in five independent fields by two independent observers in a randomized double blind fashion.
The number of immunoreactive cells in each well was counted using fluorescent microscopy in four independent fields.
Our program aims to train physicians who are excellent in two independent fields of medicine.
(Such questions are studied primarily in the independent fields of environmental ethics [see environmentalism] and animal rights).
Images were taken in ten independent fields using a Leica DM5000B fluorescence microscope.
At least 300 cells in three independent fields were counted to evaluate the presence of nuclei with apoptotic morphology.
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