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In the CSV-CLE case, metal in increasing concentration (from zero added metal) is added to a series of electrochemical cells containing the sample with the same amount of a competitive ligand.
The samples were then dehydrated in increasing concentration ethanol baths and embedded in Epon resin for 24 hours.
LPTMS was supplemented with mercury (II) (chloride) in the concentration range from 0.01 to 0.16 mM (in increasing concentration of 0.01 mM steps), copper (sulphate) in the concentration range from 3.0 to 4.2 mM (in increasing concentration of 0.1 mM steps), and (potassium) chromate in the concentration range from 0.5 to 1.0 mM (in increasing concentration of 0.1 mM steps).
MIC for methylmercury in the concentration range from 0.005 to 0.1 mM (in increasing concentration of 0.005 mM steps) was studied on paper discs on plates.
0.5 µg of either BSA, CHIP or NTD125 protein was plated per well onto which p53 (in increasing concentration) was added, followed by detection with anti-p53 antibody (PAb C-19).
Cells were fixed overnight with 2.5% glutaraldehyde at 4°C, postfixed with 1% (w/v) OsO4 for 1 h, dehydrated in increasing concentration of ethanol and embedded in Spurr's resin.
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After staining, the sections were dehydrated in increasing concentrations of ethanol and xylene.
As expected, increasing concentrations of MC in diets resulted in increasing concentrations of MC in consumers.
After observation of the vasospasm, trapidil was locally applied in increasing concentrations (10−5 10−4 M).
To confirm this, we evaluated the nucleosomal stability of dTSCs expressing H4-GFP in increasing concentrations of NaCl buffer by single-cell imaging using a microfluidic device (Fig. 5a).
Casein as the substrate was used in increasing concentrations that led to enzyme saturation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com