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In the present study, we examined genotypic differences in in vitro development and maturation in iPSC-derived neurons from AS patients and unaffected controls, from the time of initial plating up to week 20 post differentiation, considerably longer than previous examinations of iPSC-derived neurons.
The catalogs of regulated genes we have identified provide candidates for further analysis of their roles in in vitro development, and for modifying development for better control of regeneration.
Based on all this knowledge, it is not excluded that estradiol alone or in combination with other substances from the follicular fluid was involved in in vitro development of primitive oocyte-like cells in this study.
Those studies revealed a critical role for PI3Kβ in in vitro development and resorbing function of primary human and mouse osteoclasts and in in vivo bone homeostasis in experimental mice.
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Boucias, D. G., Becnel, J. J., White, S. E. & Bott, M. In vivo and in vitro development of the protist Helicosporidium sp. J Eukaryot Microbiol 48, 460 470 (2001).
Treating control neurons with UBE3A ASO at early stages of in vitro development resulted in a significant decrease in the frequency of synaptic events recorded 3 6 weeks later (Fig. 4f).
Similar to the RMP results, treatment of control neurons with UBE3A ASO at early stages of in vitro development resulted in a significantly more immature distribution of AP firing compared to scramble-treated neurons (Fig. 3c).
In the case of embryos, the in vitro development and the genotype of blastocysts were evaluated by polymerase chain reaction and sequencing.
These results indicate that 1) the ZP is unnecessary for hamster embryo development in vitro from the pronucleate ovum stage to blastocyst; 2) none of the three ZP-removal methods was detrimental to embryo development; 3) embryos do not need to be cultured in groups during in vitro development from 1-cell to blastocyst.
We found that there was no significant difference between the two groups (Table 3), which is inconsistent with the beneficial effect of CBHA in the in vitro development.
Consistent with this notion, progenitor cells of ILCs have been identified in lymph nodes of humans that could give rise to all innate lymphoid cells in the in vitro development analysis (Scoville et al., 2016).
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