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Reading them in immediate sequence put me in mind of a projector flicking successively through photo stills to form a moving picture.
Homopolymer errors were conspicuous in the column "show," which gives the immediate sequence context of the polymorphism across all strains.
Could we film the sequence twice?
In order to increase the resolution of the derived mutational signatures, substitutions were examined using their immediate sequencing context.
In addition, sample 1 was sequenced twice at variable depth.
Positive clones were selected to sequence in both directions and each sequenced twice independently.
Each product was sequenced twice in both directions using forward and reverse primers.
Three biological replicates of each treatment were analysed, with each biological replicate sequenced twice in separate lanes (sequencing replicate).
Note that the HolKor 2637 seedling was sequenced twice, and so is listed twice in this table.
Clone P2703_113 was sequenced twice with independent library preparations.
Although a letter to the company might not result in immediate changes, it might remind users to think twice about their privacy before ordering an Uber today.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com