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After vortexing, the mixture was transferred in ice to the sample cartridges on the MagNA Pure LC instrument.
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Water samples were transported in ice to the CINVESTAV-IPN laboratory in Mexico City, samples were stored at –4°C and analyzed within 1 week after collection.
The remaining sample was transported in ice to the Biochemistry Laboratory of the Yaounde University Teaching Hospital for further processing.
Samples were brought in ice to the laboratory.
Samples were stored in ice at the sampling locations and were refrigerated upon arrival in the laboratory until analyzed.
Finally, the EBC samples were transported in dry ice to the laboratory and stored at −80°C until analysis.
DNA/RNA extraction was performed in Cape Town and samples shipped on dry ice to the University of Glasgow.
Samples for RNA isolation from aerobic cultivations were taken by rapidly sampling 20 mL of culture into a tube with 35 40 mL of crushed ice in order to decrease the sample temperature to below 2°C in less than 10 seconds.
Samples for RNA isolation from two biological replicates from the late-exponential phase of glucose-limited and galactose-limited batch cultivations were taken by rapidly sampling 25 mL of culture into a 50 mL sterile Falcon tube with 40 mL of crushed ice in order to decrease the sample temperature to below 2°C in less than 10 s.
After 1-min incubation in ice, the samples were analyzed by flow cytometry.
Samples were transferred to the laboratory in coolers containing ice to reduce the degradation of samples before analysis.
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