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In human constructs, the activity was largely restricted to the first 700 bp and decreased in larger constructs.
One possible reason is that there are more TFBS in human constructs to activate gene expression or fewer inhibitors to repress gene expression.
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Nevertheless, the possibility remains that the slight variation in expression between our knock-in human construct and the mouse gene could be attributable to influence from the Hprt locus.
Self-efficacy has been viewed as a key construct in human behavior and was identified as another essential component in our measure of self-management [ 13, 14].
To address this knowledge gap, we studied how local strain and construct buckling in human TE constructs changes over culture times and GAG content.
The Isaiah vision of peace requires us to imbue our leaders with the capacity to see and act beyond the fragile human constructs in which most of us live.
However, different 6-AA NL insert sequences (LQASQT vs AEQKLT) exhibited different motile properties in both Drosophila and human constructs.
These data contrast with previous studies in which IL-1β-driven luciferase reporter activity was inhibited in a dose-dependent manner by rosiglitazone; however, those experiments were conducted in rabbit cells transiently transfected with a luciferase construct driven by the rabbit MMP-1 promoter [ 17], whereas the present investigation uses human constructs in human cells.
All of our constructs contained the same 5'-UTR (the TEV leader), but the 5'-proximal regions of the coding sequence were vastly different between the optimized and the human constructs in terms of their codon usage and GC content (Fig. 2).
We next assessed if the human constructs can function in zebrafish to rescue the MO-induced vision defect.
Furthermore, the protective effects of CNP were enhanced with stimulation by mechanical loading in human chondrocyte/agarose constructs cultured with IL-1β.
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