Sentence examples for in house vector from inspiring English sources
The GATEWAY cassette was amplified from an in house vector pDest6 using the following forward and reverse primers respectively.
The in-house vector presented here was constructed by combining commercial pUC18 and the pDONR-221 plasmid's ccdB gene encoding region; toxic gene system sequence integrity was verified by sequencing, corresponding to the expected size (659 bp).
pELMO was thus selected as an alternative choice for simplifying cloning and avoiding the occurrence of clones lacking inserts; this newly designed in-house vector is a versatile efficient tool which is suitable for cloning blunt-ended PCR-products as it relies on the effect of a toxic gene from the pDONR221 sequence.
Expression of IAPP-mCherry was achieved by adenoviral infection using an in-house vector modified to express the mCherry fluorophore.
The mrkH gene was cloned into the modified in-house vector pBAD/Myc-HisB (Invitrogen) that was made tetracycline resistant by the insertion of a tetracycline resistance gene into the ScaI site of the ampicillin resistance gene to generate plasmid pBATmrkH.
For both proteins, codon optimized genes were cloned into the multiple cloning site (SbfI, SfiI) of the in-house vector pPUZZLE containing the Zeocin resistance cassette for selection and the NTS region of the ribosomal DNA locus as genome integration sequence [ 13].
pELMO was here constructed as an in-house cloning vector for rapid and low-cost PCR product propagation; it is an optimally designed vector containing the ccdB killer gene from the pDONR 221 plasmid, cloned into the pUC18 vector's multiple cloning site (Thermo Scientific).
The in-house cloning vector thus constitutes a cheap and easy-to-use choice for general cloning and sequencing procedures.
The PCR product was cloned into an in-house pET28a vector (Novagen) modified to contain an N-terminal His8-tag.
Additional confirmation of miR-30-dependent regulation of target genes was achieved by miR-30 inhibition using an in-house sponge vector designed inhibit the entire miR-30 family.
The synthetic DI coding sequence as previously published [ 37] was cloned into an in-house modified vector that incorporates an N-terminal fusion tag consisting of an hexa-histidine motif for purification and expression, a BirA biotinylation site and a Factor Xa recognition sequence for proteolytic cleavage (Fig. 1).
