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We found no significant differences in the performance of our two "in house" methods, minisequencing with primers directly immobilised on the microarrays (Method I)[ 18] and the "tag-array" format, based on cyclic minisequencing followed by capture on microarrays using immobilised complementary "tag" probes (Method II) [ 23].
Potential NAA techniques have been evaluated in developing countries, as these methods are more affordable; these in house methods frequently use the IS 6110 element[ 7- 13].
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To have accurate results we have chosen two different methods for GYPB*S/GYPB*s genotyping: the allele-specific AS-PCR, an "in house" method, and the HEA (i.e. Human Erythrocyte Antigen) BeadChip, a microarray method commercially available.
Luckily, my questions about in-house methods for getting babies to cry prompted more mixed responses (take away a toy, take away the mother, "it just happens").
Validated against established in-house methods, the changes made to harmonize analytical protocols did not significantly affect measurement of any of the analytes.
The modified multi-analyte approaches were first validated by comparing data obtained for LS Brown snus using the approaches described above with data previously obtained for this product using established in-house methods.
The water and sediment samples collected for herbicide analysis were analysed for hexazinone, terbuthylazine and terbuthylazine-desethyl (a metabolite of terbuthylazine) by an external laboratory (RJ Hill Laboratories, Hamilton, New Zealand; http://www.hill-laboratories.com/) using in-house methods based on those of Roos et al. (1987) that are detailed in Baillie et al. (2015).
Standard investigation of solubility and permeability can be performed according to in-house methods, and the results compared with standard data sets of fasted state human intestinal fluid solubility and human effective jejunal permeability, which have been generated specifically for rDCS.
Workforces with in-house methods and means of stress-relief often have lower employee turnover rates.
Preference for in-house methods: this focus originated solely from considerations that had to do with the prospect of having to manage the resulting method for a considerable amount of time in environments of constant changes.
We used DISOPRED2 for several reasons: it was one of the best methods according to the CASP6 disorder assessment [49], it installed easily locally, and DISOPRED2 is quite orthogonal to our in-house methods [11], [59].
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