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One million reads from two different runs of in house generated ABI SOLiD sequencer data was used for all comparisons, as this is a sufficient dataset to offer reasonable comparison.
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The models were validated on an entirely different external dataset of in-house generated combinatorial library of OPs, by completely different computational approach of molecular docking against the target AChE protein of Musca domestica.
In situ hybridisation studies were conducted on paraffin embedded sections from six human eyes, using in-house generated cRNA antisense probes for human SGK1 and ENaC subunits, and confirmed expression of SGK1 and all ENaC subunits in the corneal endothelial cytoplasm.
The identification power is improved by including in-house generated reference spectra in the databases [17, 43, 46] (also see above).
The impact of capillary pressure on phase behavior was explored using an in-house generated coupled phase behavior model with a capillary pressure equation.
Using our own in-house generated PIN for the MRSA cell we have trained a boosting tree-based classifier that uses 75 physical and chemical QSAR descriptors computed for all proteins in the interaction network [2].
Anti-EKLF (5-V; in-house generated) was kindly provided by S. Phillipsen.
For the actin control the Northern blot was probed with an in-house generated mouse beta-actin probe cloned from full-length cDNA.
The MS and MS/MS raw data were processed with Bioworks 3.3.1 and searched against an in-house generated NCBI protein database using Mascot Daemon 2.2 server.
The raw MS data of the fragments from the 33-mer and the 26-mer were searched against an in-house generated database containing just these two peptides.
It contains an extensive compilation of data ranging from imported external databases to in-house generated predictions, including versions of some of the gene fusion, genome context and phylogenetic profiles methods.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com