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DLD1/Sv-Fluc transfected cells obtained from IN HOUSE CELL CULTURE COLLECTION of ICT.
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After a manager passed around copies of the magazine, an in-house cell was formed; it meets monthly in the firm's offices.
This paper experimentally and analytically investigates different design and operating parameters to enhance the performance of a PBI-based PEMFC, an in-house cell prepared in the Fuel Cell Center of Yuan Ze University.
All human cell lines were obtained from a standardized and curated in-house cell inventory (gCell; A375R1, R3 lines generated by Su et al. [10]) and grown in media either Dulbecco's modified eagle's medium (DMEM) or RPMI both supplemented with 10% of heat-inactivated FBS and 2 mM L-Glutamine (GIBCO, Life Technologies Corporation, NY, USA).
FL dose-response curves and growth inhibitory values (GIs) were generated from in-house cell growth inhibition assays.
Although bioreactor production of Influenza virus has been developed, serum-free production processes described in literature commonly use proprietary in-house cell culture media [ 24, 25, 29, 30, 32].
There is increasing evidence of pharmaceutical companies wanting to marry their in-house cell models with chemical matter developed in the academic sector, such as Eli Lilly's open innovation programme.
The NO7-152 celineise is an in-house cell line and is an early passage line grown in the traditional manner using DMEM high glucose, 4% non-essential amino acids (NEAA), 20% FCS and 2 mM GlutaMAX (all from Gibco, Carlsbad, CA, USA).
Cervical cancer cell lines (HeLa and SiHa), breast cancer cells (MCF-7 and MDA-MB-231) and monocyte cell line (THP-1) of human origin were obtained from American Type Culture Collection (Manassas, VA, USA), and maintained in our in-house cell repository, National Centre for Cell Science (NCCS), Pune, India.
Bioactive IGF was determined by an in-house, cell-based IGF-I receptor kinase activation (KIRA) assay.
Her anti-AQP4 antibody titer was relatively high as 1 8,388,608 in our in-house cell-based assay, fulfilling the 2015 diagnostic criteria of NMO spectrum disorders [ 1 ].
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