In- house calculated cut-off values regarding IgA, IgM, and IgG were 0.206, 0.325, and 0.5, respectively.
It is common to use several different software packages with results manually glued together in spreadsheets, sometimes with custom in-house calculated descriptors.
To generate figure 6 figure supplement 3, we took Illumina HT12v4 array data from 73 human iPSC lines generated in house and calculated Pluritest pluripotency and novelty scores as above, using the full probe set.
Post-processing codes are developed in-house to calculate machining forces, stress distributions, and dislocation development.
These sites were used to estimate the ratio of non-synonymous (p N ) to synonymous polymorphisms (p S ) for each transcript and each population, by using perl scripts developed in house and calculating the number of non-synonymous SNPs divided by the number of non-synonymous sites and the number of synonymous SNPs divided by the number of synonymous sites.
Electrostatic interactions were calculated using an in house program (ELECINT, R.N. Miguel, unpublished) using a dielectric constant distance dependent for electrostatic field calculation and pH = 7.4 for the calculation of charges of side chain atoms of charged residues using the Henderson-Hasselbalch equation.
For each new library, the number of compounds similar (using a similarity threshold of 0.7) to compounds within the in-house library was calculated and this number was used as a criterion for library selection (lower numbers corresponded to a high library rank).
The GMT of the International Standard Antiserum and of the in-house reference serum calculated from all measured results obtained with strain Sabin from Behringwerke were 324 and 74, respectively.
In this study, the repeatability and in-house reproducibility were calculated using samples of different feed matrices and including GMO percentages between 0.1% and 100% (Electronic supplementary material, Table S 1).
Repeatability and in-house reproducibility were calculated on the basis of the mean value of 2 × 2 PCR results (two DNA isolations and two PCR reactions with each DNA).
Fast Fourier transforms (in house routine) were calculated from 10 ms traces and spectral power density histograms were calculated with a bin of 100 Hz.
