For the determination of diosmetin glucuronides in urine, a higher quantification range was used for all three analytes: 0 1 2.667 8 24 72 210 605 1,000 ng/mL for calibration curve concentrations and 3 80 400 800 ng/mL for QC 1, 2, 3, and 4 concentrations.
We have previously compared both methods obtaining higher quantification in wastewater samples when applying the first one [ 37].
(A – A″ ) Dia protein levels are similar (∼two fold higher, quantification not shown) in tracheal cells expressing the GFP-Dia-FL construct and in the adjacent posterior spiracle cells (S).
Results from thirteen mobile phase compositions showed that the mobile phase composition of ACN/NaAcetate (5 mM /MeOH/Acetic acid (pH 3); (30:40:30; v/v/v) resulted in the highest quantification value for OTA.
For this, 4-plex iTRAQ (isobaric Tags for Relative and Absolute Quantification) is best suited to study four different samples in one single experiment with high quantification accuracy [ 15].
The MTRAP was designed and optimized on rice cell suspension telomerase extract to enable telomerase specific, reliable, and linear quantification in high throughput mode, with sensitivity comparable to those of radioisotope-based TRAP assays.
Furthermore, β-galactosidase is highly expressed in 9L tumour cells and has a very long half-life resulting in high background, which makes quantification of western blots using band density very difficult.
Dual-energy quantification systematically overestimated concentrations in lower concentration ranges and underestimated concentrations in higher concentration ranges.
Using high headline numbers (i.e. explicit and prominent quantification of maximum potential benefits) may attract participants and set higher targets that result in higher performance, but it might also lead to lower satisfaction and program abandonment.
Apart from noise, also a difference in resolution may cause the dissimilarity in quantification: higher uptake values and MRglc are found in tumours (+14%), brain (+2 to +4%) and heart (+15 to +21%) for OSEM than for FBP, which is almost completely reversible by equalizing the image resolution by smoothing with a 5-mm FWHM Gaussian kernel [ 67].
