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Analysis of peptides and proteins ranging from 1 to 8.6 kDa resulted in high resolving power single-acquisition FT-ICR mass spectra with average charge-states highly correlated to those obtained by nanoESI, thus, providing strong evidence that the ESI process dictates the observed charge-state distribution.
The strength of this method resides in high resolving power of two-dimensional electrophoresis (2-DE), coupled with highly sensitive mass spectrometry (MS), and sequence homology search.
The strength of this method resides in high resolving power of two-dimensional PAGE (2D-PAGE), coupled with polypeptide sequencing by highly sensitive mass spectrometry (MS) such as electrospray ionization tandem mass spectrometry (ESI-MS/MS), and sequence homology search in databases [ 11].
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The water distribution of a Nafion-based membrane in a direct methanol fuel cell was analyzed in-operando using high resolved synchrotron X-ray radiography.
The very high "resolving power" in the ARPES branch is achieved by using a monochromator which consists of a plane mirror and a variable line spacing grating that focuses the beam at all photon energies.
The IM-MS described in this report can achieve high resolving powers with high sensitivity, making it possible to observe and characterize low abundance isomeric species in highly complex samples with unprecedented scale and throughput.
The supernatant of each sample was loaded onto a continuous 15% - 45% (w/v) sucrose gradient in high salt resolving buffer (20 mM HEPES (pH7.4), 150 mM KCl, and 10 mM MgCl2) generated by a Biocomp gradient master.
In this case however, ion-optical aberrations must be carefully treated to reach high resolving power.
Since both deformation twinning and omega transformation occur preferably in the {211}b planes with high resolved shear stresses, it is suggested that both can be considered as alternative paths for shear transformations in shock-deformed tantalum.
A method for the regularization of LES of shock-turbulence interactions is presented which is constructed to enforce that the energy content in the highest resolved wavenumbers decays as k−5/3, and is computed locally in physical-space at low computational cost.
In this way, a conventional TWIMS separation could still be utilized in-line, with access to the higher resolving power cyclic TWIMS through orthogonal ion extraction.
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