Exact(3)
At 2 g, however, the reduced level of vimentin mRNAs might possibly be linked to the mal-adaptive down-regulation of chondrocytic genes in high intensive group.
Together with the up-regulation of hypertrophic genes in high intensive fish at 15 g, we also found increased transcription of vimentin.
ECM constituents included genes involved in bone matrix production and mineralization and 7 out of 9 of these genes were found to be down-regulated in high intensive group at 2 and 15 g.
Similar(57)
In contrast to the down-regulated transcription of osteonectin and osteocalcin, as determined by real time qPCR, we observed an increased transcription pattern of these genes in the arch centra in the high intensive group by ISH.
In proliferating chondrocytes we detected strong col2a mRNA expression in the high intensive group, but no expression in the low intensive group.
A disrupted bone and cartilage production was detected, which most likely is involved in the higher rate of deformities developed in the high intensive group.
It is not unlikely that this disequilibrium is involved in the higher rate of deformities observed in the high intensive group.
In contrast, the equivalent chondrocytes were more distorted in the high intensive group.
Moreover, an increased zone of hypertrophic chondrocytes was found in the proximity of the mineralized bone matrix in the high intensive group.
Our results showed that alp was down-regulated in the high intensive 15 g group, but up-regulated in 2 g fish.
The development from fertilization to first feeding lasted 5 months in the low intensive regime at 6°C, compared to 3 months in the high intensive regime at 10°C.
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