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On the contrary to the membrane tubulation effect which was only observed in GUV formed with the liquid disordered lipid domain, membrane bridging induced by Penetratin was observed in all four types of GUV investigated.
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Interestingly, in the case of poly-L-Lysine, a molecule without the guanidinium groups of arginine, the formation of tubes is abolished in GUV lacking anionic phospholipids [12].
The isotropic peaks observed with 31P-NMR were consistent with highly curved membranes required for the formation of tubes and vesicles as already observed in GUV.
In GUV experiments, perylene was preferentially found in lo-phases in ternary lipid mixtures containing egg sphingomyelin, DOPC and cholesterol [20].
In GUV experiments addressing cholera toxin binding to GM1 doped lipid bilayers, Baumgart et al. [20] were able to correlate the lipid domain composition and local membrane curvature proposing that these factors are important for membrane invagination processes.
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Remarkably, a similar pattern was also reproduced in GUVs.
Tension in GUVs also influences the action of AMPs, whereas the spheroplast membranes are tensionless.
However, no inner fluorescence was detected in GUVs treated with lytic peptide and calcein.
In GUVs treated with FITC-labeled lytic peptide (lytic-FITC), fluorescence increased in a time-dependent manner.
Here, we review studies of combined confocal imaging and fluorescence correlation spectroscopy (FCS) on lipid dynamics and organization in domains assembled in GUVs, prepared from various lipid mixtures, which are relevant to the problem of raft formation.
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