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The 76 bp pair-end reads were filtered for quality as described above and aligned to genomic PST assemblies using Bowtie (version 0.12.7 [ 51]) in global alignment mode allowing a maximum of two mismatches (parameters: -v 2 --best -k 1).
For individual repeat alignments HHpred submissions were made without an additional PSI-BLAST step and in global alignment mode.
CBG13231 and CBG13233 are only 54.1% identical and CBG13232 and CBG13234 are only 47.9% identical in global alignment, suggesting that these genes have diverged considerably since duplication.
In the current study, we present a refined view of the "structurally variable" regions which may contain local similarity obscured in global alignment of homologous protein structures.
This behaviour is advantageous for detection of such clusters, but may not be optimal in global alignment of sparse networks.
However, if the opposite strand of BD091237 is used in global alignment with GU591771, their similarity is 98%%.
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Overall level of similarity was calculated from percent identity in global alignments and sequence lengths.
Barker and Pagel [ 10] identified the presence/absence of each of these proteins in the fourteen other eukaryotic species, using a reciprocal best-in-genome global alignment between proteins.
If more than one contig remained in an EST-group after the two previous filtering steps, contigs within each EST-group were aligned in a global alignment using ClustalW.
First, reads that are almost fully aligned to the reference sequence (i.e. only a few bases extend past the breakpoint) can be aligned with mismatches in the global alignment portion of the BWA algorithm.
Alignment was unambiguous due to the absence of indel variation and all rbcLa sequences were readily aligned with each other in a global alignment.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com