This leads us to conclude that deeper fosmid DiTag coverage could significantly boost scaffold contiguity in future assemblies.
This assembly means that any newly composed part will adhere to its assembly standard and can be used in future assemblies [ http://dspace.mit.edu/handle/1721.1/45139].edu/handle/1721.1/45139]
In future assemblies, the incorporation of sequence data such as GSRs with EST sequences has the potential to provide more comprehensive coverage of the genes present in the tobacco genome than the EST data alone.
These could represent errors in either the de novo transcriptome assembly or the WGS assembly, and they will be investigated and corrected if necessary in future assembly releases.
Furthermore, comparative genomics through BES can be used for identifying positional candidate genes from QTL mapping studies, aid in future assembly of a reference genome sequence and elucidating sequence content and complexity in the rainbow trout genome.
However, relative to the number of mapped bases, we still have a SNP on average spaced every 2000 bases, and even non-chromosomally mapped SNPs will be useful once these segments are mapped in improved future assemblies.
The workflow used for transcriptome assembly can in future be reapplied and altered as new sequencing data becomes available for P. patula to produce a more comprehensive and complete assembly.
We identified two regions orthologous to the 11-12 duplication on the first Brachypodium genome release, confirming its presence in this species, although future assemblies using deeper coverage will be needed to confirm the chromosome locations.
Two of the DIA1L partial genes, DIA1L-pt3 and DIA1L-pt4 are similar to each other and share synteny (Table S10), and will most likely prove to be alleles of each other (and be re-annotated in a future assembly of the B. floridae genome).
In addition, a considerable amount of BAC end sequencing for Xenopus tropicalis has been done at Genoscope in France, which will support future assemblies.
However, due to the highly repetitive nature of this region, there is potential for identification of this cluster in future genome assemblies.
