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The study population was heterogeneous in functional level, symptom score, and disease stage.
Initially, cell viability was evaluated using the XF-EO maximal concentration used in functional level.
This reduction is consistent with what was observed in functional level, where maximum relaxation (100 %) was obtained in the 81 μg/mL concentration (Fig. 3a).
These results in cellular experiments support our findings in functional level and evidence that XF-EO reduce [Ca2+]c to play its spasmolytic role.
A priori sample size calculation showed that 47 patients would be sufficient to be able to detect a 0.5-unit average change in functional level from baseline with a standard deviation of 1.2 units, with 80% power, and with a 5% significance level.
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Of course, it is possible that post-transcriptional or post-translational regulatory processes could increase the variation in functional levels of these factors (both negatively and positively) in different neuronal populations.
The mRS scores may be less sensitive to changes in functional levels during hospitalisation.
All subgroups reported improvements in functional levels and in VAS pain scores, but the change was not significant in the smallest group (cartilage repair + ligament/meniscal surgery).
This may be due to reduction in functional levels as they were almost 10-11 years after surgery (average age at the time being > 80 years and majority of patients being females).
Furthermore, expression of high-risk HPV E6 has been linked to an increase in EGFR levels (Akerman et al, 2001), and changes in functional levels of the HPV E6/E7 proteins may alter the growth rate of cervical carcinoma cell lines by reducing the stability of EGFR at the posttranscriptional level (Hu et al, 1997).
As the biological activity of a protein can be regulated at post-translational level, changes in protein functional level might not be necessarily reflected by similar changes in protein abundance.
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