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2) Can CFP/YFP FRET pairs be used in FRET acceptor photobleaching experiments?
In general, when FRET occurs, the exponential decrease in FRET acceptor (YFP) signal within the bleached ROI should be accompanied by a corresponding exponential increase in FRET donor (CFP) signal following the bleach exposures [32].
Addition of nucleotide showed an appropriate anti-correlation of the donor/acceptor FRET signals and the anticipated direction of the change in FRET (increase in FRET for Inter FRET probe, and decrease in FRET for Intra FRET probe).
Then, we will account for the recent progress in FRET application based on M13 phage.
Changes in FRET measurements were compared with the release of FFAs.
In this way, changes in FRET efficiency of Ca2+ biosensors can be correlated with Ca2+ concentrations.
Purified BACE1 enzyme caused a time- and concentration-dependent linear change in FRET at low nanomolar enzyme concentrations.
Three epitopes were labeled in FRET pairs (figure 1b).
Many of these have potential for use in FRET pairs.
The strong fluorescence of piceatannol precluded its use in FRET experiments.
In most figures an initial drift in FRET ratio was corrected using exponential curve fitting.
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