Sentence examples for in frame for from inspiring English sources

The 5' end of each forward primer was designed as CACCATG in order to directionally clone it into GATEWAY destination vectors, with the ATG in frame for transcription.

Nevertheless, this deficit of SSR loci within ORF regions in EST library sequence when compared to BAC library sequence suggests that selection on actively expressed regions of the genome, particularly those in frame for translation, likely plays a significant role in the distribution of SSR loci in the Laupala genome.

GenBank accession numbers are provided in additional file 1; assembly statistics are given in additional file 5. ORFs from each strain were compared to the GenBank non-redundant protein database by BLASTX, and when two adjacent ORFs, separated by a sequence gap, were found to match adjacent fragments of the same subject protein, the ORF sequences were merged, in frame, for downstream analyses.

Orientation and codon in-frame for all constructs were confirmed by restriction analysis and sequencing.

Once the NA nucleic acid sequences were obtained, they were aligned, in-frame for protein coding, and converted to amino acids using the built-in function in BIOEDIT.

The futB gene, however, remained out of frame for all isolates passaged in wildtype mice but in-frame for four of the five tox176-passaged isolates (Figure 1 and Table 1).

Sequences were aligned with other V. cholerae sequences by using ClustalW (www.clustal.org) in MEGA4 software (17 ) and trimmed in-frame for analyses.

Eyeglasses, also called glasses or spectacles, lenses set in frames for wearing in front of the eyes to aid vision or to correct such defects of vision as myopia, hyperopia, and astigmatism.

They weren't shared with hundreds of "friends" and the few good ones would remain in frames for years.

The V5 epitope tag was in frame only for Tex19.1, Tex19.2, Tex19.1N, and Tex19.1C (Fig. 2).

In a few weeks those shirts might well be placed in a frame for safekeeping, or burned in disgust.