The purified fluorescently labeled cRNA was fragmentated in fragmentation buffer (Agilent).
mRNA was fragmented in fragmentation buffer as previously described [ 26].
Purified mRNA was then fragmented in fragmentation buffer.
Second, 20 μg of biotin-labeled cRNA was fragmented in Fragmentation Buffer.
Biotin-labelled cRNA was prepared using the Affymetrix RNA Transcript Labelling kit, and fragmented in Fragmentation Buffer (Affymetrix).
In each preparation 11 μg cRNA was fragmented in Fragmentation Buffer (Affymetrix) in a final reaction volume of 25 μl.
The mRNA was randomly fragmented by heating to 94°C in fragmentation buffer.
*The judgment will result in fragmentation of the Windows platform.
Nathaniel Stookey's "Big Bang" was a cheerful little exercise in fragmentation, silence and audience participation.
His sentences are a study in fragmentation, sometimes to brilliant effect.
The very things that began with liberal universalist dreams of common humanity ended up in fragmentation and skepticism.
