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The pellet (nuclear fraction) was washed twice in fractionation buffer, before addition of SDS-Urea loading buffer (8 M Urea, 2% SDS, 50 mM Tris HCl [pH 6.8], 0.3% bromophenol blue).
Cells were transfected using Polyfect (Qiagen) and fractionated 48 hours after transfection in fractionation buffer [10 mM Tris pH 7.5, 2.5 mM MgCl2, 10 mM DTT, PhosStop (phosphatase inhibitor cocktail; Roche), complete (protease inhibitor cocktail; Roche), 80 µg/ml Digitonin] as described (see Western blot).
The biologically effective dose (BED) of radiotherapy relative to an irradiation with a fraction dose of 2.0 Gy, that is the normalised total dose (NTD), was calculated to account for differences in fractionation according to the following formula: given the number of fractions n, the fraction size of d, and an α/β ratio of 3 Gy for telangiectasia and 2 Gy for fibrosis.
Methods for conserving energy in fractionation columns are tabulated.
For CaO/Al2O3 to vary strongly with MgO, only certain clinopyoxene compositions can be used in fractionation modelling (Fig. 4A).
Significant relationships were observed between the extent of enrichment of urine, feces, and milk, suggesting some commonality in fractionation pathways.
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This model could explain the difference in fractionations between nitrate-utilizing and N2-fixing experiments, as well as the change in fractionations with growth stage during N2 fixation.
Changes in fractionations associated with uptake and storage by these different systems could account for the differences in fractionations between A. variabilis and Azotobacter vinelandii (Table 1).
This mechanism has to explain the differences in fractionations produced with different N sources and with progressive growth during N2 fixation.
Anabaena variabilis preferentially assimilated light isotopes of Mo in all experiments, resulting in fractionations of −0.2‰ to −1.0‰ ± 0.2‰ between cells and media (εcells media), extending the range of biological Mo fractionations previously reported.
Changes in metal fractionation in the rhizospheres of unsterilized and sterilized soils were studied using rhizoboxes cultivated with seedlings of maize, wheat, pea and soybean.
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