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The AR antagonistic potency following reversed phase fractionation was 110 nmol FLU eq/g dw in fraction 3 (RP3) and 25 nmol FLU eq/g dw in fraction 4 (RP4; Fig. 4a).
Fractionation enhanced the antimicrobial activity of the crude extract in fraction F4.
These results suggest that iISGs are enriched in Fraction 6 and mISGs in Fraction 8.
A single compound was detected by LC-MS in fraction 2, 2 compounds were found in fraction 4 and a single compound in fraction 7 (Table 2).
The latter was also present in fraction R6.
As shown in Fig. 3B, the levels of insulin and p18 in mISGs were higher in Fraction 8 than those in Fraction 6, while less proinsulin and Syntaxin-6 were detected in Fraction 8 than in Fraction 6.
A single compound was detected in fraction 2 by LC-MS, while 2 compounds were found in fraction 7 (Table 1).
The content of total alkaloids in fraction C was 64.82 mg/g.
The content of total alkaloids in fraction C was determined by the bromothymol blue (BCB) [17].
The presence of the sodium adduct of xylose octanoate was identified at m/z 299.132 in fraction 99 (octanoic acid), in fraction 33 36 the fragment MX H2O was observed at m/z 259.148 (vinyl-octanoate).
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The burn-in fraction performance was evaluated using the program Tracer v1.4.
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