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To further explore factors involved in localizing the hydrogenase, we examined the distribution of HoxF-GFP fluorescence in four mutant backgrounds: the Δ hoxE and Δ hoxYH mutants lacking specific hydrogenase subunits, the hoxE deletion mutant complemented by hoxE overexpression (denoted oxhoxE hereafter), and the M55 mutant lacking respiratory Complex I.
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Rational enzyme engineering approach resulted in four mutants: G238N, E232A, D103V and Q112H.
In four mutants the alginate-overproducing TB strain had reverted to a non-mucoid phenotype (Figure 1E) and in one mutant transposon mutagenesis in a region that is homologous to a 38 kb genomic island of P. aeruginosa 2192, induced an even stronger mucoid morphotype (Figure 1H).
However, Ser/Ser phosphorylation and hence 14-3-3 14-3-3 14-3-3abindingd in four mutants (R1441G, Y1699C, E1874stop and I2020T).
Identification of the amino acid substitutions in two mutant forms of the recA protein from Escherichia coli: recA441 and recA629.
Subsequently, the location of the fluorophore was moved along the length of the protein in nine mutant proteins, and the capability to form mixed fibrils was assessed.
An irreversible oxidation signal of electro-active tyrosine (Y), tryptophan (W) and cysteine (C) residues in five mutant proteins along with the wild-type AChE were detected using square-wave voltammetry (SWV) on screen-printed carbon electrodes.
An electrophoretic mobility-shift assay and Ku antisera were used to show that Ku or a closely related protein was deficient in three mutant hamster cell lines from x-ray-sensitive complementation group 5, which is characterized by defects in DNA double-strand break repair and V(D J recombination.
Here we show that in two mutant cell lines (XR-V15B and XR-V9B) from group 5, the genetic defects are in the gene encoding the 86-kDa subunit of the Ku autoantigen, a nuclear protein that binds to the double-stranded DNA ends.
Five OsOPTs mutants had their observed phenotypes in two mutant databases.
AtBS14b expression level was analyzed, and no transcription was detected in two mutant plants (Additional file 4: Figure S3).
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