Sentence examples for in fly hemolymph from inspiring English sources
Dscam transcripts have been found in fly hemolymph, fat body cells and hemocytes [ 60].
To observe Spiroplasma in fly hemolymph, flies were dissected on microscope slides in 5 μl PBS containing 0.02 mM SYTO9 (Invitrogen, Carlsbad, CA, USA).
DOI: http://dx.doi.org/10.7554/eLife.02964.006 10.7554/eLiFigure64.007 Figure 2 figure supplement 2. Spiroplasma titers in fly hemolymph under nutrient deprivation.
To investigate the production of cardiolipin by Spiroplasma, we conducted a MALDI time-of-flight mass spectrometry (MALDI-Tof-MS) analysis of lipid species in fly hemolymph and found that there were only peaks at m/z values that correspond to cardiolipin in the hemolymph of flies-harboring Spiroplasma.
Using antibodies that recognize such proteins, Schmucker's postdoc, Fiona Watson, found the molecules in fruit fly hemolymph--the insect equivalent of blood serum--and on the surfaces of fat body cells and immune cells called hemocytes.
Extracts from flies without expression of TTR (GMR-Gal4; +) were used as the negative controls in the toxicity assays, since we observed increased cell viability in cultures treated with fly extract (probably associated with the presence of fly hemolymph proteins), compared to cells growing with medium only.
We observed that expressing the destabilized F57I and D67H lysozymes triggers UPR activation, resulting in degradation of these variants, whereas the WT lysozyme is secreted into the fly hemolymph.
While insulin promotes the uptake of sugar by tissues from the blood (hemolymph in flies) and its storage as glycogen and fats, AKH is secreted during starvation conditions to break down glycogen and fats [ 17].
Importantly, we observed that Spiroplasma proliferation in whole flies and hemolymph was also significantly inhibited when flies are maintained on a nutrient poor diet.
For example, fundamental differences between the hemolymph system in flies and the mammalian vasculature and circulatory system bring into question the utility of this organism to accurately model metastatic processes.
In addition, Figure 4C, which analyzes hemolymph glucose levels in flies fed water rather than molasses food after the primary injury, shows that the increase in glucose levels is due to food ingested after the primary injury, further supporting the conclusion that intestinal dysfunction is a secondary injury.
