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In fly head homogenates, specific immunoprecipitation of dP32-EGFP was observed.
We detected full-length human APP in fly head lysates from both transgenic backgrounds when induced with elav-Gal4 (Fig. 1).
The pathway interactions of dSir2 and Dmp53 were confirmed at the biochemical level by showing through co-immunoprecipitation experiments in fly head lysates that Dmp53 and Sir2 physically interact with each other.
The highest non-toxic, final concentration of ZDON in the food was found to be 125 µM, and this concentration was then tested for its ability to inhibit TG2 activity in fly head homogenates using an in vitro TG activity assay.
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In order to quantify the increase of Cu levels in flies raised on copper-supplemented food, we used ICP-MS to measure copper in fly heads.
Expression of milton RNAi in neurons with the pan-neuronal elav-GAL4 driver reduced the mRNA levels of milton in fly heads (Figure 3A), and resulted in 60% reduction in milton protein levels in dissected fly brains (Figure 3B).
Expression of Aβ42 does not affect the steady state levels of copper in fly heads, which contain less than one third of copper compared to the rest of the body (Fig. S2B).
We also found that GstD1, InR and Hsp70 expression exhibits an age-dependent increase in fly heads; however their relative expression levels are significantly affected by NF using different numbers of reference genes.
A number of laboratories have reported that changes in white (w) function (codes for an ABC transporter protein) can have effects on male-male courtship [34] [37], male aggression [38], learning [39] and memory [26], and also can cause changes in the levels of monoamines in fly heads [26], [40].
Memory-inducing training generates a transient pulse of Csw-dependent Ras-MAPK signaling in fly heads.
Microarray profiling has shown that PTZ exerts a downregulatory effect on gene expression in fly heads.
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