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The raw reads that were high quality with Phred Quality Values > Q20 (ASCII Character Code +33) on basis of Sanger Quality were used to analyze genetic variations in five accessions.
The present data show sexual reproduction in five accessions of the eastern Alps.
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Further, accession-specific markers were observed in eight accessions.
To get the specific variation information, we compared the potential SNPs in four accessions with the dbSNP.
We sequenced the entire vvd gene in eight accessions and identified 24 nucleotide changes and six amino acid changes in the coding region (Figure S4).
Here, SNPs were retained only if the reference (Matina 1-6) appearedppeared in two accessions and the alternative allele also appeared in two accessions.
Ratios measuring the relative amount of 26,090 70-mer sequences in two accessions' genomes were derived in the following way.
Analysis of sequence mutations was done to study the polymorphism of NPR1 in fifteen accessions of the Vitaceae family.
We found the previously described VRN-A1f allele in four accessions of T. monococcum (Table 2; Additional file 1).
PCR analysis of the 1st intron region revealed allele VRN-A1L in three accessions of T. dicoccoides.
Pm3c was the most frequently detected allele in this set (found in 17 accessions), followed by Pm3b in six accessions while Pm3f, Pm3e and Pm3d were detected in two, two and one accessions each, respectively.
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