Sentence examples for in final concentration from inspiring English sources

Reactions were started by the addition of glycerol, vitamin B12 (1.5 μM in final concentration; Sigma-Aldrich Jandn, Tokyo, Japan), and 50 mM KCl into the equal parts of the cell suspension.

For the latter treated and untreated bacterial cells were incubated for 60 min at 37 °C with MTT dye (3- 4,5-dimethylthiazolyl-2 -2,5-diphenyltetrazolium bromide, M2128-1G, Sigma-Aldrich) in final concentration 0.05 mg/ml.

Alternatively, the protoplasts were isolated from infiltrated tobacco leaves, and incubated with 0, 0.25 or 0.5 μM NADPH in final concentration for 5 min, and subsequently the protoplasts were imaged using a confocal microscope.

Fifty microliters of bacterial suspension (in final concentration 2.5 × 105 CFU/ml) was added to the latter tube, and tubes were agitated at 300 rpm for 24 h at 37 ° C. The CFUs/ml of test wells was calculated using Miles and Misra method [26].

The effect of punicic acid was dose dependent starting from 10 µM up to 40 µM in final concentration (Figure D).

Biotinylated peptides were pre-incubated 2 h at 100 µM or 200 µM (in final concentration with lysate) at room temperature with 30 µl of streptavidin-coated immunomagnetic beads (Calbiochem, San Diego CA).

Penicillin and streptomycin were then added in final concentrations of 100 units/ml and 100 µg/ml, respectively (Mediatech).

TCN (Biaffin GmbH, Kassel, Germany) was dissolved in DMSO to prepare a 3 mM stocksolution and was used in final concentrations of 1 and 5 µM.

The drugs were added in final concentrations between 0.1 and 50 microM.

Cystathionine (Cth) was used in final concentrations of 1 and 3 mM.

Therefore, Ficoll and Dextran in final concentrations ranging from 5%to15%% are better crowding agents for accelerating HDA.