Moreover, it will also help testers to devote time in exploratory test which requires manual efforts.
The administration of RU 24969 stimulated locomotion in the free exploratory test in both strains, but RU 24969 produced opposite effects on anxiety in the two strains, suggesting that while the drug affects locomotion, it may not affect the expression of anxiety.
This was not found in the present study; in the free exploratory test, bicuculline had no effect on locomotion or rearing in either strain.
But no consistent evidence of an anxiogenic profile has been found [79] and it has been suggested that when anxiogenesis is observed after bicuculline administration, it may be attributed to behavioural suppression rather than to any effect on anxiety; for example, a dose of 1 mg/kg produced behavioural suppression in Swiss mice in the free exploratory test [80].
Bicuculline induced anxiety in B6 mice in both the free exploratory test (a decrease in the time spent in the novel environment) and the light/dark test, but induced an anxiolytic effect on ABP mice in the elevated plus-maze.
In the free exploratory test, ABP mice spent more time in the novel side, suggesting they are less anxious (table 3).
In my opinion, exploratory testing will continue to be an integral part of testing and will never be replaced with AI.
The behaviour cannot be linked to any difference in the level of locomotion, as not only were there no strain-related differences for entries into the closed arms, but in the free exploratory test, the general activity of B6 mice was higher than ABP mice.
In exploratory analysis test results were examined by site, by age, and by nutritional status.
In addition, a reduction of exploratory rearing was consistently found in both the novel-cage exploratory test (t(7) = −2.408, p = 0.047; Fig. 2C) and rat-cage behavior test (t(7) = −5.043, p = 0.001; Fig. 2E) of TTC9A−/− female mice as compared to the TTC9A+/+ animals.
